<b>Isolation of chlorophyll <i>a</i> from spinach leaves</b>
نویسندگان
چکیده
منابع مشابه
Localization of Chlorophyll in Spinach Chloroplast Lamellae by Fluorescence Microscopy
Chlorophyll in green plants is thought to reside in the internal membranes or lamellae of chloroplasts (1). These membranes are observable by electron microscopy and can be roughly grouped, on the basis of size and arrangement , into grana lamellae and stroma lamellae which exist as closed, flattened sacs, or thylakoids (2). Al though the chlorophyll molecule is not yet demonstrable by electron...
متن کاملRegulated Chlorophyll Degradation in Spinach Leaves during Storage
Degradation of chlorophyll in spinach (Spinacia olearacea L. cv. Hybrid 612) appeared to be regulated through the peroxidase-hydrogen peroxide pathway, which opens the porphyrin ring, thus resulting in a colorless compound. This conclusion was arrived at from the analysis of chlorophylls (Chls) and their metabolizes by HPLC and of enzyme activities catalyzing the degradative reactions. Chls dec...
متن کاملATP-induced chlorophyll luminescence in isolated spinach chloroplasts.
Upon a light-dark transition chloroplasts emit post-illumination luminescence [l] , which is commonly considered to result from the recombination of positive charges (Z’) and negative charges (Q-) at the photosystem II reaction centers [ 1,2] . Stimulation of post-illumination luminescence by a variety of treatments has been reported (for a recent review, see [3]) such as transthylakoidal ApH [...
متن کاملIsolation and Characterization of a New Minor Chlorophyll a/b-Protein Complex (CP24) from Spinach.
We have identified a new minor chlorophyll a/b-protein complex in the thylakoid membranes of spinach (Spinacia oleracea L.), which migrates as a green band below CPII on mildly denaturing polyacrylamide gels. This complex, designated CP24, was isolated from octyl glucoside/sodium dodecyl sulfate solubilized spinach grana membrane fractions by preparative gel electrophoresis and has been charact...
متن کاملOrgan-specific distribution of chlorophyll-related compounds from dietary spinach in rabbits.
The distribution of chlorophyll-related compounds (CRCs) derived from dietary spinach was investigated in different organs the rabbits. The rabbits in the experimental group consumed 100 g of freeze-dried spinach powder after a 24 h fasting period and sacrificed 2, 4, 8, 12 and 24 h later and in the control group sacrificed after the 24 h fasting period. The main CRCs in the liver were found to...
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ژورنال
عنوان ژورنال: Bulletin of the Chemical Society of Ethiopia
سال: 2008
ISSN: 1011-3924
DOI: 10.4314/bcse.v22i2.61302